Hetzer, Martin; Meyer, Hemmo; Walther, Tobias C.; Bilbao-Cortes, Daniel; Warren, Graham; Mattaj, Iain W.:
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
In: Nature Cell Biology, Jg. 3 (2001), Heft 12, S. 1086 - 1091
2001Artikel/Aufsatz in Zeitschrift
Biologie
Damit verbunden: 1 Publikation(en)
Titel:
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
Autor*in:
Hetzer, Martin;Meyer, HemmoUDE
GND
1089446616
LSF ID
51479
ORCID
0000-0003-1883-1796ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Walther, Tobias C.;Bilbao-Cortes, Daniel;Warren, Graham;Mattaj, Iain W.
Erscheinungsjahr:
2001

Abstract:

Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.