Hetzer, Martin; Meyer, Hemmo; Walther, Tobias C.; Bilbao-Cortes, Daniel; Warren, Graham; Mattaj, Iain W.:
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
2001
In: Nature Cell Biology, Jg. 3 (2001), Heft 12, S. 1086 - 1091
Artikel/Aufsatz in Zeitschrift2001Biologie
Titel:
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
Autor(in):
Hetzer, Martin; Meyer, HemmoLSF; Walther, Tobias C.; Bilbao-Cortes, Daniel; Warren, Graham; Mattaj, Iain W.
Erscheinungsjahr
2001
WWW URL

Abstract:

Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.