Hetzer, Martin; Meyer, Hemmo; Walther, Tobias C.; Bilbao-Cortes, Daniel; Warren, Graham; Mattaj, Iain W.:

Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly

In: Nature Cell Biology, Jg. 3 (2001) ; Nr. 12, S. 1086 - 1091
ISSN: 1465-7392
Zeitschriftenaufsatz / Fach: Biologie
Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.