Alam, Steven L; Sun, Ji; Payne, Marielle; Welch, Brett D; Blake, B Kelly; Davis, Darrell R; Meyer, Hemmo; Emr, Scott D; Sundquist, Wesley I:
Ubiquitin Interactions of NZF Zinc Fingers
2004
In: EMBO Journal, Jg. 23 (2004), Heft 7, S. 1411 - 1421
Artikel/Aufsatz in Zeitschrift / Fach: Biologie
Titel:
Ubiquitin Interactions of NZF Zinc Fingers
Autor(in):
Alam, Steven L; Sun, Ji; Payne, Marielle; Welch, Brett D; Blake, B Kelly; Davis, Darrell R; Meyer, Hemmo im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Emr, Scott D; Sundquist, Wesley I
Erscheinungsjahr:
2004
Erschienen in:
EMBO Journal, Jg. 23 (2004), Heft 7, S. 1411 - 1421
ISSN:
Link URL:

Abstract:

Ubiquitin (Ub) functions in many different biological pathways, where it typically interacts with proteins that contain modular Ub recognition domains. One such recognition domain is the Npl4 zinc finger (NZF), a compact zinc-binding module found in many proteins that function in Ub-dependent processes. We now report the solution structure of the NZF domain from Npl4 in complex with Ub. The structure reveals that three key NZF residues (13TF14/M25) surrounding the zinc coordination site bind the hydrophobic 'Ile44' surface of Ub. Mutations in the 13TF14/M25 motif inhibit Ub binding, and naturally occurring NZF domains that lack the motif do not bind Ub. However, substitution of the 13TF14/M25 motif into the nonbinding NZF domain from RanBP2 creates Ub-binding activity, demonstrating the versatility of the NZF scaffold. Finally, NZF mutations that inhibit Ub binding by the NZF domain of Vps36/ESCRT-II also inhibit sorting of ubiquitylated proteins into the yeast vacuole. Thus, the NZF is a versatile protein recognition domain that is used to bind ubiquitylated proteins during vacuolar protein sorting, and probably many other biological processes.