Knauer, Shirley; Carra, Gert; Stauber, Roland H.:
Nuclear Export Is Evolutionarily Conserved in CVC Paired-Like Homeobox Proteins and Influences Protein Stability, Transcriptional Activation, and Extracellular Secretion.
2005
In: Molecular and cellular biology : MCB, Jg. 6 (2005), Heft 7, S. 594 - 606
Artikel/Aufsatz in Zeitschrift / Fach: Biologie
Titel:
Nuclear Export Is Evolutionarily Conserved in CVC Paired-Like Homeobox Proteins and Influences Protein Stability, Transcriptional Activation, and Extracellular Secretion.
Autor(in):
Knauer, Shirley im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Carra, Gert; Stauber, Roland H.
Erscheinungsjahr:
2005
Erschienen in:
Molecular and cellular biology : MCB, Jg. 6 (2005), Heft 7, S. 594 - 606
ISSN:
Link URL:

Abstract:

Homeodomain transcription factors control a variety of essential cell fate decisions during development. To understand the developmental regulation by these transcription factors, we describe here the molecular analysis of paired-like CVC homeodomain protein (PLC-HDP) trafficking. Complementary experimental approaches demonstrated that PLC-HDP family members are exported by the Crm1 pathway and contain an evolutionary conserved leucine-rich nuclear export signal. Importantly, inactivation of the nuclear export signal enhanced protein stability, resulting in increased transactivation of transfected reporters and decreased extracellular secretion. In addition, PLC-HDPs harbor a conserved active nuclear import signal that could also function as a protein transduction domain. In our study, we characterized PLC-HDPs as mobile nucleocytoplasmic shuttle proteins with the potential for unconventional secretion and intercellular transfer. Nucleocytoplasmic transport may thus represent a conserved control mechanism to fine-tune the transcriptional activity of PLC-HDPs prerequisite for regulating and maintaining the complex expression pattern during development.