De Cian, Anne; Guittat, Lionel; Kaiser, Markus; Saccà, Barbara; Amrane, Samir; Bourdoncle, Anne; Alberti, Patrizia; Teulade-Fichou, Marie-Paule; Lacroix, Laurent; Mergny, Jean-Louis:

Fluorescence-based melting assays for studying quadruplex ligands

In: Methods : A Companion to Methods in Enzymology, Jg. 42 (2008) ; Nr. 2, S. 183-195
ISSN: 1046-2023
Zeitschriftenaufsatz / Fach: Biologie
Abstract:
The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to directly inhibit telomerase activity. The reactivation of this enzyme in immortalized and most cancer cells suggests that telomeres and telomerase are relevant targets in oncology, and telomere ligands and telomerase inhibitors have been proposed as new potential anticancer agents. In this paper, we have analysed the FRET method used to measure the stabilization and selectivity of quadruplex ligands towards the human telomeric G-quadruplex. The stabilization value depends on the nature of the fluorescent tags, the incubation buffer, and the method chosen for Tm calculation, complicating a direct comparison of the results obtained by different laboratories.