Amrane, Samir; De Cian, Anne; Rosu, Frédéric; Kaiser, Markus; De Pauw, Edwin; Teulade-Fichou, Marie-Paule; Mergny, Jean-Louis:
Identification of CNG trinucleotide repeat ligands with a FRET melting assay.
2008
In: ChemBioChem : a European journal of chemical biology, Jg. 9 (2008), Heft 8, S. 1229 - 1234
Artikel/Aufsatz in Zeitschrift2008Biologie
Titel:
Identification of CNG trinucleotide repeat ligands with a FRET melting assay.
Autor(in):
Amrane, Samir; De Cian, Anne; Rosu, Frédéric; Kaiser, MarkusLSF; De Pauw, Edwin; Teulade-Fichou, Marie-Paule; Mergny, Jean-Louis
Erscheinungsjahr
2008
WWW URL
Erschienen in:
Titel:
ChemBioChem : a European journal of chemical biology
in:
Jg. 9 (2008), Heft 8, S. 1229 - 1234
ISSN:
Signatur der UB

Abstract:

DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligands that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.