Amrane, Samir; De Cian, Anne; Rosu, Frédéric; Kaiser, Markus; De Pauw, Edwin; Teulade-Fichou, Marie-Paule; Mergny, Jean-Louis:
Identification of CNG trinucleotide repeat ligands with a FRET melting assay.
2008
In: ChemBioChem : a European journal of chemical biology, Jg. 9 (2008), Heft 8, S. 1229 - 1234
Artikel/Aufsatz in Zeitschrift / Fach: Biologie
Titel:
Identification of CNG trinucleotide repeat ligands with a FRET melting assay.
Autor(in):
Amrane, Samir; De Cian, Anne; Rosu, Frédéric; Kaiser, Markus im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; De Pauw, Edwin; Teulade-Fichou, Marie-Paule; Mergny, Jean-Louis
Erscheinungsjahr:
2008
Erschienen in:
ChemBioChem : a European journal of chemical biology, Jg. 9 (2008), Heft 8, S. 1229 - 1234
ISSN:
Signatur der UB:
Link URL:

Abstract:

DNA hairpin structures formed within a repeated tract might be a causative factor for triplet expansion observed in several debilitating diseases. We have designed and used a fluorescence resonance energy transfer (FRET) melting assay to screen for ligands that bind specifically to the CNG triplet repeats. Using this assay, we screened a panel of 33 chemicals that were previously designed to bind DNA or RNA secondary structures. Remarkably, we found that macrocyclic compounds, such as acridine dimers and trimers, exhibit interesting affinities and specificities for this motif.