Rose, Rolf; Huttenlocher, Franziska; Cedzich, Anna; Kaiser, Markus; Schaller, Andreas; Ottmann, Christian:

Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.

In: Acta crystallographica, Section F, Structural biology and crystallization communications online, Jg. 65 (2009) ; Nr. 5, S. 522–525
Zeitschriftenaufsatz / Fach: Biologie
Fakultät für Biologie
Abstract:
The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.