Rose, Rolf; Huttenlocher, Franziska; Cedzich, Anna; Kaiser, Markus; Schaller, Andreas; Ottmann, Christian:
Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
2009
In: Acta crystallographica, Section F, Structural biology and crystallization communications online, Jg. 65 (2009), Heft 5, S. 522 - 525
Artikel/Aufsatz in Zeitschrift2009Biologie
Fakultät für Biologie
Titel:
Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
Autor(in):
Rose, Rolf; Huttenlocher, Franziska; Cedzich, Anna; Kaiser, MarkusLSF; Schaller, Andreas; Ottmann, Christian
Erscheinungsjahr
2009
WWW URL

Abstract:

The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.