Mondigler, Martin; Ehrmann, Michael:
Site-specific proteolysis of the Escherichia coli SecA protein in vivo
1996
In: Journal of bacteriology : JB, Jg. 178 (1996), Heft 10, S. 2986 - 2988
Artikel/Aufsatz in Zeitschrift / Fach: Biologie
Fakultät für Biologie
Titel:
Site-specific proteolysis of the Escherichia coli SecA protein in vivo
Autor(in):
Mondigler, Martin; Ehrmann, Michael im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen
Erscheinungsjahr
1996
Erschienen in:
Journal of bacteriology : JB, Jg. 178 (1996), Heft 10, S. 2986 - 2988
ISSN
Signatur der UB
WWW URL

Abstract:

A seven-amino-acid cleavage site specific for tobacco etch virus (TEV) protease was introduced into SecA at two separate positions after amino acids 195 and 252. Chromosomal wild-type secA was replaced by these secA constructs. Simultaneous expression of TEV protease led to cleavage of both SecA derivatives. In the functional SecA dimer, proteolysis directly indicated surface exposure of the TEV protease cleavage sites. Cleavage of SecA near residue 195 generated an unstable proteolysis product and a secretion defect, suggesting that this approach could be used to inactivate essential proteins in vivo.