Ehrle, Rainer; Mikhaleva, Natasha; Boyd, Dana; Davidson, Amy L.; Ehrmann, Michael:

Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions

In: Research in Microbiology, Jg. 154 (2003) ; Nr. 9, S. 654-657
ISSN: 0923-2508
Zeitschriftenaufsatz / Fach: Biologie
ehem. Fakultät für Biologie und Geografie
Charged residues were introduced into the C-terminal transmembrane segment of a MalF–PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.