Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
In: Research in Microbiology, Jg. 154 (2003) ; Nr. 9, S. 654-657
Zeitschriftenaufsatz / Fach: Biologie
ehem. Fakultät für Biologie und Geografie
Charged residues were introduced into the C-terminal transmembrane segment of a MalF–PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.