Ehrle, Rainer; Mikhaleva, Natasha; Boyd, Dana; Davidson, Amy L.; Ehrmann, Michael:
Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
2003
In: Research in Microbiology, Jg. 154 (2003), Heft 9, S. 654 - 657
Artikel/Aufsatz in Zeitschrift2003Biologie
Fakultät für Biologie
Titel:
Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
Autor(in):
Ehrle, Rainer; Mikhaleva, Natasha; Boyd, Dana; Davidson, Amy L.; Ehrmann, MichaelLSF
Erscheinungsjahr
2003
WWW URL

Abstract:

Charged residues were introduced into the C-terminal transmembrane segment of a MalF–PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.