Hauske, Patrick; Mamant, Nicolette; Hasenbein, Sonja; Nickel, Sabrina; Ottmann, Christian; Clausen, Tim; Ehrmann, Michael; Kaiser, Markus:
Peptidic small molecule activators of the stress sensor DegS
2009
In: Molecular BioSystems, Jg. 5 (2009), Heft 9, S. 980 - 985
Artikel/Aufsatz in Zeitschrift / Fach: Biologie
Fakultät für Biologie
Titel:
Peptidic small molecule activators of the stress sensor DegS
Autor(in):
Hauske, Patrick im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Mamant, Nicolette; Hasenbein, Sonja; Nickel, Sabrina im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Ottmann, Christian; Clausen, Tim; Ehrmann, Michael im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Kaiser, Markus
Erscheinungsjahr:
2009
Erschienen in:
Molecular BioSystems, Jg. 5 (2009), Heft 9, S. 980 - 985
ISSN:
Link URL:

Abstract:

Bacterial DegS is a regulatory protease that acts as a molecular stress sensor and initiates a periplasmic stress response pathway. Upon binding of misfolded proteins to its PDZ domain, the protease domain of DegS is allosterically activated, thereby initiating a signal cascade that results in the elevated expression of protein quality control factors. Although the structural basis of this activation mode has been elucidated previously, it is not yet fully understood if binding to the PDZ domain is sufficient for protease domain activation or if secondary interactions with the protease domain are required. Here, we demonstrate that tripeptidic small molecule activators which only bind to the PDZ domain are sufficient to trigger DegS activation . Furthermore, we show that the hydrophobicity of the peptidic small molecule activators is a critical determinant for efficient activation .