Hallmann, A; Wodniok, Sabina:

Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa

In: Plant Cell Reports, Jg. 25 (2006) ; Nr. 6, S. 582-591
ISSN: 1432-203X, 0721-7714
Zeitschriftenaufsatz / Fach: Biologie
Fakultät für Biologie » Allgemeine Botanik
Abstract:
Production of transgenic organisms is a well-established, versatile course of action in molecular biology. Genetic engineering often requires heterologous, dominant  antibiotic resistance genes that have been used as  selectable markers  in many species. However, as heterologous 5' and 3' flanking sequences  often result in very low expression rates, endogenous flanking sequences, especially promoters, are mostly required and are easily obtained in  model organisms, but it is much more complicated and time-consuming to get appropriate sequences from less common organisms. In this paper, we show that aminoglycoside 3'-phosphotransferase gene  (aphVIII) based  constructs with  3' and 5' untranslated flanking sequences (including promoters) from the multicellular green alga Volvox work in the unicellular green  alga Chlamydomonas and flanking sequences  from Chlamydomonas work in Volvox, at least if a low expression rate is compensated by an enforced high gene dosage. This strategy might be useful for all investigators that intend to transform species in which genomic sequences are not available, but sequences from related organisms exist.

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