Quantitative determination of the composition of multi-shell calcium phosphate-oligonucleotide nanoparticles and their application for the activation of dendritic cells

In: Acta Biomaterialia, Jg. 7 (2011) ; Nr. 11, S. 4029–4036
ISSN: 1878-7568
Zeitschriftenaufsatz / Fach: Chemie
Fakultät für Chemie » Anorganische Chemie
Abstract:
Biodegradable previous termcalciumnext termprevious termphosphatenext termprevious termnanoparticlesnext term as carriers for the immunoactive toll-like receptor ligands CpG and polyinosinic–polycytidylic acid for the previous termactivationnext term of previous termdendriticnext termprevious termcellsnext term (DC) combined with the viral antigen hemagglutinin (HA) were prepared. A purification method based on ultracentrifugation and ultrasonication was developed to separate the previous termnanoparticlesnext term from dissolved biomolecules. The number of biomolecules, i.e., oligonucleotides and peptide, incorporated into the previous termnanoparticlesnext term was quantitatively determined by UV-spectroscopy, using fluorescent derivatives of the biomolecules. The immunostimulatory effects of purified previous termcalciumnext termprevious termphosphatenext termprevious termnanoparticlesnext term on DC were studied, i.e., cytokine production and previous termactivationnext term of the previous termcellsnext term in terms of the upregulation of surface molecules. Purified previous termcalciumnext termprevious termphosphatenext termprevious termnanoparticlesnext term, i.e., without dissolved biomolecules, are capable of inducing adaptive immunity by previous termactivationnext term of DC. Immunostimulatory effects of purified previous termcalciumnext termprevious termphosphatenext termprevious termnanoparticlesnext term on DC were demonstrated by increased expression of co-stimulatory molecules and MHC II and by cytokine secretion. In addition, DC treated with purified functionalized previous termcalciumnext termprevious termphosphatenext termprevious termnanoparticlesnext term induced an antigen-specific T-previous termcellnext term response in vitro.