Computational Design of a DNA- and Fc-Binding Fusion Protein
In: Advances in Bioinformatics, Band 2011, S. 457578
2011Artikel/Aufsatz in Zeitschrift
BiologieInformatikFakultät für BiologieForschungszentren » Zentrum für Medizinische Biotechnologie (ZMB)
Damit verbunden: 1 Publikation(en)
Titel:
Computational Design of a DNA- and Fc-Binding Fusion Protein
Autor*in:
Winkler, JonasUDE
- LSF ID
- 51903
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
- LSF ID
- 49401
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
- LSF ID
- 49787
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
- LSF ID
- 50610
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
Erscheinungsjahr:
2011
Abstract:
Computational design of novel proteins with well-defined functions is an ongoing topic in computational biology. In this work, we generated and optimized a new synthetic fusion protein using an evolutionary approach. The optimization was guided by directed evolution based on hydrophobicity scores, molecular weight, and secondary structure predictions. Several methods were used to refine the models built from the resulting sequences. We have successfully combined two unrelated naturally occurring binding sites, the immunoglobin Fc-binding site of the Z domain and the DNA-binding motif of MyoD bHLH, into a novel stable protein.