Jost, Steffen Oliver; Medinger, Ralph; Boenigk, Jens:
Cultivation-independent species identification of dinobryon species (Chrysophyceae) by means of multiplex single-cell Pcr1
2010
In: Journal of Phycology, Jg. 46 (2010), Heft 5, S. 901 - 906
Artikel/Aufsatz in Zeitschrift2010Biologie
Fakultät für Biologie » Allgemeine Botanik
Titel:
Cultivation-independent species identification of dinobryon species (Chrysophyceae) by means of multiplex single-cell Pcr1
Autor(in):
Jost, Steffen OliverLSF; Medinger, Ralph; Boenigk, JensLSF
Erscheinungsjahr
2010
WWW URL
Erschienen in:
Titel:
Journal of Phycology
in:
Jg. 46 (2010), Heft 5, S. 901 - 906
ISSN:

Abstract:

With the discovery of a high molecular diversity of protists, a discrepancy between morphological and molecular species richness estimates became apparent. Solving the current concerns requires a comparative analysis of different sequences combined with morphological analyses of single cells originating from preserved field samples. We refined a single-cell PCR (SC-PCR) protocol for analyzing cells from field samples preserved with Lugol’s iodine solution. We linked microscopic screening with multiplex PCR targeting the SSU rDNA, internal transcribed spacer 1 (ITS1), 5.8S rDNA, internal transcribed spacer 2 (ITS2), and the mitochondrial cytochrome oxidase 1 (CO1) in a single PCR reaction. Using this method, we investigated the intraspecific molecular variation in Dinobryon populations originating from two lakes in the Salzkammergut area of Austria. All investigated genetic markers showed two separated clusters within the investigated populations of Dinobryon divergens O. E. Imhof, indicating a reproductive isolation of the two coexisting populations. Based on these findings, we describe a lineage, which is morphologically similar to D. divergens but, based on the molecular data, is reproductively isolated.