Haferkamp, Patrick; Kutschki, Simone; Treichel, Jenny; Hemeda, Hatim; Sewczyk, Karsten; Hoffmann, Daniel; Zaparty, Melanie; Siebers, Bettina:
An additional glucose dehydrogenase from Sulfolobus solfataricus: fine-tuning of sugar degradation?
2011
In: Biochemical Society Transactions, Jg. 39 (2011), Heft 1, S. 77 - 81
Artikel/Aufsatz in Zeitschrift / Fach: Chemie; Biologie; Medizin
Titel:
An additional glucose dehydrogenase from Sulfolobus solfataricus: fine-tuning of sugar degradation?
Autor(in):
Haferkamp, Patrick im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Kutschki, Simone; Treichel, Jenny; Hemeda, Hatim; Sewczyk, Karsten im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Hoffmann, Daniel im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Zaparty, Melanie im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Siebers, Bettina im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen
Erscheinungsjahr:
2011
Erschienen in:
Biochemical Society Transactions, Jg. 39 (2011), Heft 1, S. 77 - 81
ISSN:
ISSN:
Signatur der UB:
Link URL:

Abstract:

Within the SulfoSYS (Sulfolobus Systems Biology) project, the effect of temperature on a metabolic network is investigated at the systems level. Sulfolobus solfataricus utilizes an unusual branched ED (Entner-Doudoroff) pathway for sugar degradation that is promiscuous for glucose and galactose. In the course of metabolic pathway reconstruction, a glucose dehydrogenase isoenzyme (GDH-2, SSO3204) was identified. GDH-2 exhibits high similarity to the previously characterized GDH-1 (SSO3003, 61% amino acid identity), but possesses different enzymatic properties, particularly regarding substrate specificity and catalytic efficiency. In contrast with GDH-1, which exhibits broad substrate specificity for C5 and C6 sugars, GDH-2 is absolutely specific for glucose. The comparison of kinetic parameters suggests that GDH-2 might represent the major player in glucose catabolism via the branched ED pathway, whereas GDH-1 might have a dominant role in galactose degradation via the same pathway as well as in different sugar-degradation pathways.