GLUCOSE CATABOLISM OF THE HYPERTHERMOPHILIC ARCHAEUM THERMOPROTEUS-TENAX

In: FEMS MICROBIOLOGY LETTERS, Jg. 111 (1993) ; Nr. 1, S. 1-8
ISSN: 0378-1097
Zeitschriftenaufsatz / Fach: Biologie
Abstract:
Pulse labelling experiments and enzyme studies show that Thermoproteus tenax is able to degrade glucose via the Embden-Meyerhof-Parnas (EMP) pathway. T tenax is the first archaeum for which the glycolytic EMP pathway could be established. One of the key enzymes, 6-phosphofructokinase of T tenax depends on pyrophosphate (pyrophosphate-fructose-6-phosphate-1-phosphotransferase) instead of ATP as found in some bacterial and eucaryal species. In addition to the intermediates of the EMP pathway the intermediary products of the non-phosphorylated Entner-Doudoroff pathway were also detected by pulse labelling experiments indicating that under chemoorganotrophic conditions at least 2 glycolytic pathways are operative in T tenax.