Analysis of aminobenzoic acids in waste water from a former ammunition plant with HPLC and combined diode array and fluorescence detection
In: Fresenius' Journal of Analytical Chemistry, Fresenius J. Anal. Chem., Jg. 357 (1997) ; Nr. 1, S. 121-126
Zeitschriftenaufsatz / Fach: Chemie
Aminobenzoic acids are of major interest in clinical analysis, but their determination in environmental samples has been rarely done. Therefore a method for the determination of 10 aminobenzoic acids in water has been developed, using high- performance liquid chromatography with combined diode array and fluorescence detection. A separation of the analytes has been achieved with a buffer pH 3/methanol gradient. The general elution order has been m-substituted < p-subst. < o-subst. aminobenzoic acids. UV and fluorescence data are presented. In a highly polluted water sample of the former ammunition plant Stadtallendorf/Hessen, 4-aminobenzoic acid, 2-aminobenzoic acid and 2-amino-4-nitrobenzoic acid have been detected. After passing a charcoal filter, only the concentration of 4- aminobenzoic acid in a sample from the same site has been above the detection limit. Besides the ten analytes investigated, several unknown compounds have been present in the samples.