Jensen, Manfred; Feige, Guido B.; Waterkotte, Anna:

Mannitol-1-Phosphate dehydrogenase in Pseudevernia furfuracea

In: Lichenologist, Jg. 23 (1991) ; Nr. 2, S. 187-196
Zeitschriftenaufsatz / Fach: Biologie
Abstract:
Mannitol-1-phosphate dehydrogenase [EC 1.1.1.17] activity was demonstrated in extracts of Pseudevernia furfuracea and Hypogymnia physodes. The reaction was found to be highly substrate specific for fructose-6-phosphate/NADH or mannitol-1-phosphate/NAD+. The pH optimum for fructose-6-phosphate reduction was 7.1, and apparent Km values were 1.2 mM for fructose-6-phosphate and 20 μM for NADH. The reaction did not require Mg++ or Ca++. For conversion of mannitol-1-phosphate into mannitol, the occurrence of mannitol-1-phosphatase in Pseudevernia furfuracea is postulated.