Eser, Markus; Henrichs, Tanja; Boyd, Dana; Ehrmann, Michael:
Target directed proteolysis in vivo.
2007
In: Methods in Enzymology, Band 421 (2007), S. 68 - 83
Artikel/Aufsatz in Zeitschrift2007Biologie
Titel:
Target directed proteolysis in vivo.
Autor(in):
Eser, Markus; Henrichs, Tanja; Boyd, Dana; Ehrmann, MichaelLSF
Erscheinungsjahr
2007
Erschienen in:
Titel:
Methods in Enzymology
in:
Band 421 (2007), S. 68 - 83
ISSN:

Abstract:

The experimental problems associated with in vivo studies of essential proteins or integral membrane proteins have triggered geneticists to generate novel approaches that have often led to insights of general relevance (Shuman and Silhavy, 2003). In order to extend the experimental portfolio, we developed target-directed proteolysis (TDP), an in vivo method allowing structural and functional characterization of target proteins in living cells. TDP is based on the activity of the highly sequence-specific NIa protease from tobacco etch virus. When its recognition site of seven residues is engineered into target proteins and NIa protease is expressed under tight promoter control, substrates can be conditionally processed while other cellular proteins remain unaffected. Applications include conditional inactivation as well as functional characterization of target proteins.