Adam, Vojtech; Zehnalek, Josef; Petrlova, Jitka; Potesil, David; Sures, Bernd; Trnkova, Libuse; Jelen, Frantisek; Vitecek, Jan; Kizek, Rene:

Phytochelatin modified electrode surface as a sensitive heavy metal ion biosensor.

In: Sensors, Jg. 5 (2005) ; Nr. 1, S. 70-84
ISSN: 1424-8220
Zeitschriftenaufsatz / Fach: Biologie
Electrochemical biosensors have superior properties over other existing
measurement systems because they can provide rapid, simple and low-cost on-field
determination of many biological active species and a number of dangerous pollutants. In
our work, we suggested a new heavy metal biosensor based on interaction of heavy metal
ions (Cd2+ and Zn2+) with phytochelatin, which was adsorbed on the surface of the hanging
mercury drop electrode, using adsorptive transfer stripping differential pulse voltammetry.
In addition, we applied the suggested technique for the determination of heavy metals in a
biological sample – human urine and platinum in a pharmaceutical drug. The detection
limits (3 S/N) of Cd(II), Zn(II) and cis-platin were about 1.0, 13.3 and 1.9 pmole in 5 μl,
respectively. On the basis of the obtained results, we propose that the suggested technique
offers simple, rapid, and low-cost detection of heavy metals in environmental, biological
and medical samples.