Ehrenhofer-Murray, Ann; Keller Seitz, M. U.; Sengstag, C.:

The Sge1 protein of Saccaharomyces cerevisiae is a membrane-associated multidrug transporter

In: Yeast : a forum for yeast researchers, Jg. 14 (1998) ; Nr. 1, S. 49-65
ISSN: 0749-503X
Zeitschriftenaufsatz / Fach: Chemie
In this study, we report the further characterization of the
   Saccharomyces cerevisiae crystal violet-resistance protein Sge1. Sge1 is
   a highly hydrophobic 59 kDa protein with 14 predicted membrane-spanning
   domains. It shares homologies with several drug-resistance proteins and
   sugar transporters of the major facilitator superfamily. Here, we have
   demonstrated that Sge1 is not only a crystal violet-resistance protein,
   but that it also confers resistance to ethidium bromide and
   methylmethane sulfonate. Disruption of SGE1 leads to increased
   sensitivity towards all three compounds, thus designating Sge1 as a
   multiple drug-resistance protein. Subcellular fractionation as well as
   immunolocalization on whole yeast cells demonstrated that Sge1 was
   tightly associated with the yeast plasma membrane. Furthermore, Sge1 was
   highly enriched in preparations of yeast plasma membranes. In analogy to
   other multidrug-resistance proteins, we suggest that Sge1 functions as a
   drug export permease