Marchfelder, Uta; Rateitschak, Katja; Ehrenhofer-Murray, Ann:

SIR-dependent repression of non-telomeric genes in Saccharomyces cerevisiae?

In: Yeast : a forum for yeast researchers, Jg. 20 (2003) ; Nr. 9, S. 797-801
ISSN: 0749-503X
Zeitschriftenaufsatz / Fach: Chemie
The proteins Sir2, Sir3 and Sir4 repress transcription of the silent mating-type loci
HML and HMR and of reporter genes inserted at telomeres. Previous microarray
analyses suggested that additional non-telomeric genes exist which are repressed by
the Sir proteins. In this study, we tested the expression of 12 such genes by Northern
analysis and RT–PCR. However, we were unable to verify their SIR-dependent
regulation, which suggests that SIR-mediated repression may be restricted to the
known repressed regions