Gluesenkamp, Karl Heinz; Krueger, Kai; Eberle, Gertrud; Drosdziok, Wolfgang; Jaehde, Eckhard; Gruendel, Oliver; Neuhaus, Andrea; Boese, Roland; Stellberg, Peter; Rajewsky, Manfred E.:
Tautomer-specific monoclonal antibodies against N-3-substituted adenines as new tools for molecular epidemiology.
1993
In: Angewandte Chemie, Jg. 105 (1993), Heft 11, S. 1710 - 1712
Artikel/Aufsatz in Zeitschrift / Fach: Chemie
Titel:
Tautomer-specific monoclonal antibodies against N-3-substituted adenines as new tools for molecular epidemiology.
Autor(in):
Gluesenkamp, Karl Heinz; Krueger, Kai; Eberle, Gertrud; Drosdziok, Wolfgang; Jaehde, Eckhard; Gruendel, Oliver; Neuhaus, Andrea; Boese, Roland im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen; Stellberg, Peter; Rajewsky, Manfred E. im Online-Personal- und -Vorlesungsverzeichnis LSF anzeigen
Erscheinungsjahr:
1993
Erschienen in:
Angewandte Chemie, Jg. 105 (1993), Heft 11, S. 1710 - 1712
ISSN:

Abstract:

Tautomer-specific monoclonal antibodies (mab) to N-3-substituted adenines were generated, which bound the modified adenines with a high affinity regardless of the size of the groups on position 3 of the adenine. N-3-substituted haptens were prepd. and bound to a carrier protein, then used to induce an immune response. Mabs with affinity consts. for 3-ethyladenine in the region of 1010 L/mol were generated. The antibodies demonstrated a high specificity for N-3-substituted adenine derivs. These mabs might be useful in the purifn. of N-3-substituted adenines from body fluids or in the monitoring of chemotherapy with alkylating cytostatic agents.