Gluesenkamp, Karl Heinz; Krueger, Kai; Eberle, Gertrud; Drosdziok, Wolfgang; Jaehde, Eckhard; Gruendel, Oliver; Neuhaus, Andrea; Boese, Roland; Stellberg, Peter; Rajewsky, Manfred E.:

Tautomer-specific monoclonal antibodies against N-3-substituted adenines as new tools for molecular epidemiology.

In: Angewandte Chemie, Jg. 105 (1993) ; Nr. 11, S. 1710-12
ISSN: 0044-8249
Zeitschriftenaufsatz / Fach: Chemie
Abstract:
Tautomer-specific monoclonal antibodies (mab) to N-3-substituted adenines were generated, which bound the modified adenines with a high affinity regardless of the size of the groups on position 3 of the adenine. N-3-substituted haptens were prepd. and bound to a carrier protein, then used to induce an immune response. Mabs with affinity consts. for 3-ethyladenine in the region of 1010 L/mol were generated. The antibodies demonstrated a high specificity for N-3-substituted adenine derivs. These mabs might be useful in the purifn. of N-3-substituted adenines from body fluids or in the monitoring of chemotherapy with alkylating cytostatic agents.