Isolation and biochemical characterization of extracellular polymeric substances from Pseudomonas aeruginosa.

In: Methods in enzymology (Meth.Enzymol.), Jg. 336 (2001) ; Microbial Growth in Biofilms, Part A, S. 302-314
ISSN: 0076-6879
Zeitschriftenaufsatz / Fach: Chemie
In general, the extracellular polymeric substances (EPS) of P. aeruginosa predominantly consist of different polysaccharides and proteins. Detailed methods for isolation and biochem. characterization are described for both whole EPS and alginate from mucoid strains of P. aeruginosa grown as a confluent bacterial lawn on agar media as simple in vitro model systems for bacterial biofilms. (c) 2001 Academic Press.