Polythionate metabolism in Thiomonas intermedia K12.
In: Process Metallurgy, Jg. 9A (1999) ; Biohydrometallurgy and the Environment Toward the Mining of the 21st Century, Pt. A, S. 787-797
Zeitschriftenaufsatz / Fach: Chemie
Metab. of thiosulfate and tetrathionate by Thiomonas intermedia K12, a facultatively heterotrophic, reduced sulfur compds.-oxidizing bacterium, was investigated. Expts. with the inhibitors N-ethylmaleimide (NEM), myxothiazol, and cyanide, and, addnl., the protonophore carbonylcyanide m-chlorophenolhydrazone (CCCP) were performed in short-time expts. to elucidate the degrdn. pathways. The results indicate a linked, cyclic degrdn. of these compds. via the highly reactive key intermediate disulfane-monosulfonic acid. Furthermore, anaerobic degrdn. expts., starting with tetrathionate, revealed a stoichiometry, which indicates a disproportionation of tetrathionate to thiosulfate, trithionate, and sulfate. The disproportionation allowed for cell growth.