Transport and reaction behavior of invertase ultrafiltration membranes made from substituted polysulfone.
Ultrafiltration membranes were made from bromomethylated polysulfone (BrPSf) with varying degree of substitution. They were used to bind invertase. The enzyme fixation was performed in such a way that it was incorporated within the membrane matrix. The kinetic behavior of the enzyme-loaded membranes was detd. using sucrose. The effective kinetic parameters of the reaction were obtained under diffusion-controlled and convection-controlled conditions. The diffusion expts. enabled the evaluation of the enzyme distribution within the membrane. From the balance of different educt/product ratios as a function of residence time the activity of the transferase catalyzed reaction could be derived.
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