New method for determination of â-lactam antibiotics by means of Diffuse Reflectance Spectroscopy using polyurethane foam as sorbent

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The present study was focused to develop an analytical method to analyse â-lactam antibiotics present in aqueous solutions and able to be applied directly in the fields. The method here presented is based on the chemical reaction between â-lactam antibiotics with phosphomolybdic acid, a heteropolyacid able to be reduced by the thiols obtained after the acid hydrolysis of the â-lactam antibiotics. The blue product obtained, which is proportional to the antibiotic amount, is adsorbed on polyurethane foam, and the intensity of color is directly evaluated on the solid material by means of diffuse reflectance spectroscopy. For all six evaluated â-lactam antibiotics (amoxicillin, ampicillin, penicillin V, penicillin G, cefotaxime, cefuroxime), the analytical parameters achieved were: LOD = 0.03 µg/ml, LOQ = 0.1 µg/ml, RSD = 4.0 %. The study of possible interferences showed that using solid phase extraction is possible to eliminate even strong interferences as ascorbic acid, cysteine and reducing salts like stannous chloride. The present method has avoided, as much as possible, the use of expensive and contaminating solvents and chemical reagents. The method presents the possibility to be applied using only a desktop scanner and a personal PC for measuring the color of the adsorbed material on the PUF.
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Dokumententyp:
Wissenschaftliche Abschlussarbeiten » Dissertation
Fakultät / Institut:
Fakultät für Chemie
Dewey Dezimal-Klassifikation:
500 Naturwissenschaften und Mathematik » 540 Chemie
Stichwörter:
antibiotics, â-lactam antibiotics, sorption mechanisms, antimicrobial drug resistance, Kubelka Munk, solid phase extraction, phosphomolybdic acid, heteropolyacids, preconcentration, adsorption isotherms, sorption, diffuse reflectance spectroscopy, ampicillin, amoxicillin, polyurethane foam, penicillin, heteropoly blues
Beitragende:
Priv.-Doz. Dr. Kuß, Heinz-Martin [Betreuer(in), Doktorvater]
Dr. Denkhaus, Evelin [Gutachter(in), Rezensent(in)]
Sprache:
Deutsch
Kollektion / Status:
Dissertationen / Dokument veröffentlicht
Datum der Promotion:
13.12.2005
Dokument erstellt am:
13.12.2005
Dateien geändert am:
13.12.2005
Medientyp:
Text